Dr Dipti Pitta from the School of Veterinary Medicine at the University of Pennsylvania has requested cultures or DNA from Methanogenic archaeon ISO4-H5 and Methanobrevibacter AbM4. Dr Pitta has recently submitted a paper quantifying a panel of methanogens in rumen samples collected from dairy cows supplemented with 3-NOP and red seaweed using quantitative PCR, and has been asked by reviewers of the paper to use DNA from pure cultures of rumen methanogens to verify her results. The methanogenic archaeon ISO4-H5 and Methanobrevibacter AbM4 are not available from commercial culture collections, so Dr Pitta has approached use to supply these strains. The strains will not be used for commercial purposes, only for verification of their qPCR data.