The sequence data to be cleared in this approval will be deposited with EBI as a pre-requisite for submitting a planned manuscript for publication:
Henning Seedorf, Sandra Kittelmann, Gemma Henderson, Peter H. Janssen "Analysis of New Zealand ruminant methanogenic archaea using a refined taxonomic framework". The manuscript is not being submitted for approval yet. We first need to confirm the datasets that will be allowed to be deposited because these will determine what can be analysed for the content of the manuscript.
The subject of this approval is sequence data generated from archaeal 16S rRNA genes amplified from DNA of rumen samples collected during the following PGgRc-funded trials (no reference will be made in the data to be deposited regarding methane measurements or treatment groups):

1. Cesar Pinares’s 40 sheep (grass and pellet-fed animals)
2. Vaccine Trial 1 (non-vaccinated control group only)
3. Vaccine Trial 2 (non-vaccinated control group only). 

Once the datasets are finalised, then the final analyses can be performed, and the manuscript completed and submitted through the ROI process.

The sequencing data need to deposited in publicly available resources for gene sequences (e.g. NCBI or EBI) prior to publication. This is a common policy for international peer-reviewed journals and the processing of this kind of sequence data deposition can take several months. Due to the duration of this process and in order not to delay publication, deposition of the data prior to publication should be initiated early.
