Sequence data will be submitted to SRA to obtain accession numbers, which will then be included into a draft manuscript. The manuscript will compare pyrosequencing and microscopic counting of rumen ciliate protozoa to assess rumen ciliate community structure in the rumen (manuscript will be submitted for PGgRc-NZAGRC approval separately at a later stage). The sequence data covered in this ROI will not be released by NCBI SRA until the final manuscript is accepted by a journal. 