
The student intern (Andreas Schielke) is leaving soon and has written a thesis.

The thesis covers only thermophilic enzymes.

He targeted the following (and got these results):
1.	The GTPcyclo, CA enzymes for crystallography (not much progress). We have worked with the rumen bug enzymes for screening. We were hoping to use student data to be able to publish the thermophilic enzymes at some point. There are already papers out on these enzymes which I can provide to you if you like.
2.	The G1P from Methanopyrus which grows at 98C for X-tals and characterisation (similar to the recently OK’ed MJ G1P-which is now in revision at PNAS). He got a pH optimum and salt optimum.
3.	Another enzyme that we mention in the G1P paper is called GGGPS from Methanothermobacter (there are already crystals that have been published for this enzyme in two papers, but no one yet has the one of the substrates in the active site). We have not made an attempt yet with that-but will with Vince’s Marsden if we get it...
4.	He also targeted another enzyme in lipid synthesis (DGGGPS) but got no expression.
5.	MptA (first step methanopterin synthesis) and CitG (methanopterin synthesis) from Methanothermobacter (got expression for both but got no further). We already had expression for CitG from ruminantium, but the diffraction was never very good. 
