Dear Mark

Tim McAllister’s group are putting together a manuscript entitled “Considerations in the application of fluorescence microscopy to define spatial distributions and characterise rumen methanogens” that they plan to submit to FEMS Microbial Ecology. We could potentially contribute some PGgRc microbial ecology programme-derived data on the optimisation of FISH (fluorescent in situ hybridisation) with methanogens to this manuscript. This PGgRc-funded work involved carrying out FISH with various methanogens with and without prior treatment with the enzyme peiW (the peiW FISH method has been published – see reference below). We found that peiW treatment improved FISH with methanogens in some cases.   I have submitted the report that contains the relevant data to the ROI system. As far as I can tell, no IP is associated with  this work. I propose to acknowledge the PGgRc as follows for its contribution to this work in the manuscript: Work on fluorescent in situ hybridization with peiW was carried out at AgResearch under contract to the Pastoral Greenhouse Gas Research Consortium (PGgRc; www.pggrc.co.nz). I’d appreciate if you could approve it for release and inclusion in Tim’s manuscript as soon as convenient. Tim or I will provide you with a copy of the manuscript in due course.  Please get in touch with myself or Peter should you have any queries.

Best wishes,

Gemma






Nakamura K, Terada T, Sekiguchi Y, Shinzato N, Meng XY, Enoki M, Kamagata Y (2006) Application of pseudomurein endoisopeptidase to fluorescence in situ hybridization of methanogens within the family Methanobacteriaceae. Applied and Environmental Microbiology 72:6907-6913
